Title : Relationship of reduced folate changes to inhibition of DNA synthesis induced by methotrexate in L1210 cells in vivo.

Pub. Date : 1989 Aug 1

PMID : 2743307






1 Functional Relationships(s)
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1 A radioenzymatic assay based upon entrapment of tissue 5,10-methylenetetrahydrofolate (CH2FH4), and other reduced folates after cycling to this form, into a stable ternary complex with thymidylate synthase and [3H]-5-fluoro-2"-deoxyuridine 5"-monophosphate was used to estimate reduced folates. Folic Acid thymidylate synthase Mus musculus