Title : An experimental method for the determination of enzyme-competitive inhibitor dissociation constants from displacement curves: application to human renin using fluorescence energy transfer to a synthetic dansylated inhibitor peptide.

Pub. Date : 1989 Aug 15

PMID : 2683862






2 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 The procedure involves binding a dansylated competitive inhibitor, U80215, followed by its displacement by an unlabeled inhibitor of renin. U 80215 renin Homo sapiens
2 Binding of U80215 is monitored by fluorescent energy transfer from the renin tryptophans to the dansyl moiety; displacement of U80215 by an unlabeled inhibitor is monitored by a reversal of this process. U 80215 renin Homo sapiens