Title : Evidence That Compound I Is the Active Species in Both the Hydroxylase and Lyase Steps by Which P450scc Converts Cholesterol to Pregnenolone: EPR/ENDOR/Cryoreduction/Annealing Studies.

Pub. Date : 2015 Dec 8

PMID : 26603348






5 Functional Relationships(s)
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1 Evidence That Compound I Is the Active Species in Both the Hydroxylase and Lyase Steps by Which P450scc Converts Cholesterol to Pregnenolone: EPR/ENDOR/Cryoreduction/Annealing Studies. Pregnenolone cytochrome P450 family 11 subfamily A member 1 Homo sapiens
2 Cytochrome P450scc (CYP 11A1) catalyzes the conversion of cholesterol (Ch) to pregnenolone, the precursor to steroid hormones. Pregnenolone cytochrome P450 family 11 subfamily A member 1 Homo sapiens
3 Cytochrome P450scc (CYP 11A1) catalyzes the conversion of cholesterol (Ch) to pregnenolone, the precursor to steroid hormones. Pregnenolone cytochrome P450 family 11 subfamily A member 1 Homo sapiens
4 This procedure is validated by showing that the cryoreduced ternary complexes of oxy-cytochrome P450scc with HC and DHC are catalytically competent and during annealing generate DHC and pregnenolone, respectively. Pregnenolone cytochrome P450 family 11 subfamily A member 1 Homo sapiens
5 In combination with our previous results, this study thus indicates that Cpd I is the active species in each of the three sequential monooxygenation reactions by which P450scc catalytically converts Ch to pregnenolone. Pregnenolone cytochrome P450 family 11 subfamily A member 1 Homo sapiens