Pub. Date : 2016 Jan 1
PMID : 26476415
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Cytoprotective role of autophagy against BH3 mimetic gossypol in ATG5 knockout cells generated by CRISPR-Cas9 endonuclease. | Gossypol | autophagy related 5 | Homo sapiens |
| 2 | The MTT assay revealed that the inhibitory effect of gossypol was weaker on ATG5 knockout cells than that on the wild type (WT) cells. | Gossypol | autophagy related 5 | Homo sapiens |
| 3 | However, Cyto-ID autophagy assay revealed that gossypol induced ATG5- and LC3-independent autophagy in ATG5 knockout cells. | Gossypol | autophagy related 5 | Homo sapiens |
| 4 | However, Cyto-ID autophagy assay revealed that gossypol induced ATG5- and LC3-independent autophagy in ATG5 knockout cells. | Gossypol | autophagy related 5 | Homo sapiens |
| 5 | Moreover, gossypol acts as an autophagy inducer in ATG5 knockout cells while blocking the later stages of the autophagy process in WT cells, which was determined by measuring autophagic flux after co-treatment of gossypol with chloroquine (late-stage autophagy inhibitor). | Gossypol | autophagy related 5 | Homo sapiens |
| 6 | On the other hand, inhibition of autophagy with 3-MA or Beclin-1 siRNA caused a partial increase in the sensitivity to gossypol in ATG5 knockout cells, but not in the WT cells. | Gossypol | autophagy related 5 | Homo sapiens |
| 7 | Together, our findings suggest that the resistance to gossypol in ATG5 knockout cells is associated with increased cytoprotective autophagy, independent of ATG5. | Gossypol | autophagy related 5 | Homo sapiens |