Pub. Date : 2015
PMID : 26464590
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Expression level analysis indicated that PTEN reached its peak level at 36( )C for 8 h with 1.5625mM IPTG, while solubility analysis revealed the optimal induction temperature was at 20( )C, the optimal IPTG concentration was 0.1microM and the optimal induction time was up to 8 h. Taken together, we provide an optimal induction condition for expressing soluble fusion protein of PTEN in E. coli, facilitating further analysis of PTEN"s biological function in vitro. | Isopropyl Thiogalactoside | phosphatase and tensin homolog | Homo sapiens |
| 2 | Expression level analysis indicated that PTEN reached its peak level at 36( )C for 8 h with 1.5625mM IPTG, while solubility analysis revealed the optimal induction temperature was at 20( )C, the optimal IPTG concentration was 0.1microM and the optimal induction time was up to 8 h. Taken together, we provide an optimal induction condition for expressing soluble fusion protein of PTEN in E. coli, facilitating further analysis of PTEN"s biological function in vitro. | Isopropyl Thiogalactoside | phosphatase and tensin homolog | Homo sapiens |
| 3 | Expression level analysis indicated that PTEN reached its peak level at 36( )C for 8 h with 1.5625mM IPTG, while solubility analysis revealed the optimal induction temperature was at 20( )C, the optimal IPTG concentration was 0.1microM and the optimal induction time was up to 8 h. Taken together, we provide an optimal induction condition for expressing soluble fusion protein of PTEN in E. coli, facilitating further analysis of PTEN"s biological function in vitro. | Isopropyl Thiogalactoside | phosphatase and tensin homolog | Homo sapiens |
| 4 | Expression level analysis indicated that PTEN reached its peak level at 36( )C for 8 h with 1.5625mM IPTG, while solubility analysis revealed the optimal induction temperature was at 20( )C, the optimal IPTG concentration was 0.1microM and the optimal induction time was up to 8 h. Taken together, we provide an optimal induction condition for expressing soluble fusion protein of PTEN in E. coli, facilitating further analysis of PTEN"s biological function in vitro. | Isopropyl Thiogalactoside | phosphatase and tensin homolog | Homo sapiens |