Title : IFNγ+ Treg in-vivo and in-vitro represent both activated nTreg and peripherally induced aTreg and remain phenotypically stable in-vitro after removal of the stimulus.

Pub. Date : 2015 Aug 13

PMID : 26268522






4 Functional Relationships(s)
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1 When phorbol 12-myristate 13-acetate (PMA)/Ionomycin was washed out from the cell culture after 6 h stimulation, Treg induction continued for at least 96 h of cell culture, contradicting the hypothesis that removal of the stimulus results in significant decrease of IFNgamma- and IFNgamma+ CD4+CD25+Foxp3+CD127- Treg due to loss of Foxp3 expression. Tetradecanoylphorbol Acetate forkhead box P3 Homo sapiens
2 When phorbol 12-myristate 13-acetate (PMA)/Ionomycin was washed out from the cell culture after 6 h stimulation, Treg induction continued for at least 96 h of cell culture, contradicting the hypothesis that removal of the stimulus results in significant decrease of IFNgamma- and IFNgamma+ CD4+CD25+Foxp3+CD127- Treg due to loss of Foxp3 expression. Tetradecanoylphorbol Acetate forkhead box P3 Homo sapiens
3 When phorbol 12-myristate 13-acetate (PMA)/Ionomycin was washed out from the cell culture after 6 h stimulation, Treg induction continued for at least 96 h of cell culture, contradicting the hypothesis that removal of the stimulus results in significant decrease of IFNgamma- and IFNgamma+ CD4+CD25+Foxp3+CD127- Treg due to loss of Foxp3 expression. Tetradecanoylphorbol Acetate forkhead box P3 Homo sapiens
4 When phorbol 12-myristate 13-acetate (PMA)/Ionomycin was washed out from the cell culture after 6 h stimulation, Treg induction continued for at least 96 h of cell culture, contradicting the hypothesis that removal of the stimulus results in significant decrease of IFNgamma- and IFNgamma+ CD4+CD25+Foxp3+CD127- Treg due to loss of Foxp3 expression. Tetradecanoylphorbol Acetate forkhead box P3 Homo sapiens