Title : Overexpression of ZWF1 and POS5 improves carotenoid biosynthesis in recombinant Saccharomyces cerevisiae.

Pub. Date : 2015 Oct

PMID : 26179622






2 Functional Relationships(s)
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1 When ZWF1 was overexpressed (Sc-EYBIZH+I), glucose-6-phosphate dehydrogenase activity increased by 103-fold, the transcription level of crtE and crtI increased significantly, the lycopene and beta-carotene yield increased to 2 29 +- 0 06 and 0 38 +- 0 02 mg l(-1) respectively. Lycopene glucose-6-phosphate dehydrogenase Saccharomyces cerevisiae S288C
2 When ZWF1 and POS5 were overexpressed in a carotenoid-producing S. cerevisiae strain individually, the total yield of lycopene and beta-carotene increased by 59 9% and 81 4%, respectively, and the final product beta-carotene yield increased by 18 8% and 65 6% respectively. Lycopene glucose-6-phosphate dehydrogenase Saccharomyces cerevisiae S288C