Pub. Date : 2015 Feb 25
PMID : 25880059
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | A recombinant CYP11B1 dependent Escherichia coli biocatalyst for selective cortisol production and optimization towards a preparative scale. | Hydrocortisone | cytochrome P450 family 11 subfamily B member 1 | Homo sapiens |
| 2 | CYP11B1 thus offers a great potential for biotechnological application in large-scale synthesis of cortisol. | Hydrocortisone | cytochrome P450 family 11 subfamily B member 1 | Homo sapiens |
| 3 | For the subsequent optimization of the whole-cell activity 3 different approaches were pursued: Firstly, CYP11B1 expression was enhanced 3.3-fold to 257 nmol*L(-1) by site-directed mutagenesis of position 23 from glycine to arginine, which was accompanied by a 2.6-fold increase in cortisol yield. | Hydrocortisone | cytochrome P450 family 11 subfamily B member 1 | Homo sapiens |