Title : Sequence requirements for cytochrome P-450IIB1 catalytic activity. Alteration of the stereospecificity and regioselectivity of steroid hydroxylation by a simultaneous change of two hydrophobic amino acid residues to phenylalanine.

Pub. Date : 1989 Dec 15

PMID : 2574176






2 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Although the published sequences of IIB1 and IIB2 are identical within the N-terminal halves of the proteins, sequence analysis of the variant cDNA revealed two amino acid substitutions in this region; Leu58----Phe and I1e114----Phe. Phenylalanine cytochrome P450 family 2 subfamily B member 6 Homo sapiens
2 A IIB1 protein that possessed only the I1e114----Phe replacement catalyzed the production of all four testosterone metabolites with only slightly different product ratios compared with the parent enzyme. Phenylalanine cytochrome P450 family 2 subfamily B member 6 Homo sapiens