Title : Probing the electrostatics of active site microenvironments along the catalytic cycle for Escherichia coli dihydrofolate reductase.

Pub. Date : 2014 Jul 23

PMID : 24977791






1 Functional Relationships(s)
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1 Herein, the changes in active site electrostatic microenvironments are examined for all enzyme complexes along the catalytic cycle of Escherichia coli dihydrofolate reductase (ecDHFR) by incorporation of thiocyanate probes at two site-specific locations in the active site. thiocyanate dihydrofolate reductase Escherichia coli