Pub. Date : 2014 Jun
PMID : 24915100
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Preliminary joint X-ray and neutron protein crystallographic studies of ecDHFR complexed with folate and NADP+. | NADP | dihydrofolate reductase | Escherichia coli |
| 2 | A crystal of Escherichia coli dihydrofolate reductase (ecDHFR) complexed with folate and NADP+ of 4x1.3x0.7 mm (3.6 mm3) in size was obtained by sequential application of microseeding and macroseeding. | NADP | dihydrofolate reductase | Escherichia coli |
| 3 | The neutron and X-ray data were used together for joint refinement of the ecDHFR-folate-NADP+ ternary-complex structure in order to examine the protonation state, protein dynamics and solvent structure of the complex, furthering understanding of the catalytic mechanism. | NADP | dihydrofolate reductase | Escherichia coli |