Pub. Date : 2014 Jul
PMID : 24760895
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We recently reported that herpes simplex virus 1 (HSV-1) protein kinase Us3 phosphorylated viral dUTPase (vdUTPase) at serine 187 (Ser-187) to upregulate its enzymatic activity, which promoted HSV-1 replication in human neuroblastoma SK-N-SH cells but not in human carcinoma HEp-2 cells. | Serine | Deoxyuridine triphosphatase | Drosophila melanogaster |
| 2 | We recently reported that herpes simplex virus 1 (HSV-1) protein kinase Us3 phosphorylated viral dUTPase (vdUTPase) at serine 187 (Ser-187) to upregulate its enzymatic activity, which promoted HSV-1 replication in human neuroblastoma SK-N-SH cells but not in human carcinoma HEp-2 cells. | Serine | Deoxyuridine triphosphatase | Drosophila melanogaster |
| 3 | Furthermore, the replacement of Ser-187 in vdUTPase with aspartic acid, which mimics constitutive phosphorylation, and overexpression of cellular dUTPase restored viral replication to the wild-type level in cellular dUTPase knockdown HEp-2 cells. | Serine | Deoxyuridine triphosphatase | Drosophila melanogaster |
| 4 | These results indicated that sufficient dUTPase activity was required for efficient HSV-1 replication and supported the hypothesis that Us3 phosphorylation of vdUTPase Ser-187 upregulated vdUTPase activity in host cells with low cellular dUTPase activity to produce efficient viral replication.virus. | Serine | Deoxyuridine triphosphatase | Drosophila melanogaster |
| 5 | These results indicated that sufficient dUTPase activity was required for efficient HSV-1 replication and supported the hypothesis that Us3 phosphorylation of vdUTPase Ser-187 upregulated vdUTPase activity in host cells with low cellular dUTPase activity to produce efficient viral replication.virus. | Serine | Deoxyuridine triphosphatase | Drosophila melanogaster |