Title : Zinc finger nuclease-mediated knockout of AHR or ARNT in human breast cancer cells abolishes basal and ligand-dependent regulation of CYP1B1 and differentially affects estrogen receptor α transactivation.

Pub. Date : 2014 Mar

PMID : 24299737






5 Functional Relationships(s)
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1 Basal and TCDD-, 17beta-estradiol (E2)-, or TCDD + E2-dependent recruitment of AHR, ARNT, ERalpha, NCoA3, and RNA polymerase II to CYP1B1 as well as CYP1B1 mRNA levels were abolished in MCF7-AHR((ko)) and MDA-MB-231 AHR(ko) cells. Polychlorinated Dibenzodioxins cytochrome P450 family 1 subfamily B member 1 Homo sapiens
2 Basal and TCDD-, 17beta-estradiol (E2)-, or TCDD + E2-dependent recruitment of AHR, ARNT, ERalpha, NCoA3, and RNA polymerase II to CYP1B1 as well as CYP1B1 mRNA levels were abolished in MCF7-AHR((ko)) and MDA-MB-231 AHR(ko) cells. Polychlorinated Dibenzodioxins cytochrome P450 family 1 subfamily B member 1 Homo sapiens
3 Basal and TCDD-, 17beta-estradiol (E2)-, or TCDD + E2-dependent recruitment of AHR, ARNT, ERalpha, NCoA3, and RNA polymerase II to CYP1B1 as well as CYP1B1 mRNA levels were abolished in MCF7-AHR((ko)) and MDA-MB-231 AHR(ko) cells. Polychlorinated Dibenzodioxins cytochrome P450 family 1 subfamily B member 1 Homo sapiens
4 Basal and TCDD-, 17beta-estradiol (E2)-, or TCDD + E2-dependent recruitment of AHR, ARNT, ERalpha, NCoA3, and RNA polymerase II to CYP1B1 as well as CYP1B1 mRNA levels were abolished in MCF7-AHR((ko)) and MDA-MB-231 AHR(ko) cells. Polychlorinated Dibenzodioxins cytochrome P450 family 1 subfamily B member 1 Homo sapiens
5 Our data show that AHR and ARNT play critical roles in the basal, TCDD, and E2-induced regulation of CYP1B1 but also reveal distinct roles for both proteins in ERalpha transactivation. Polychlorinated Dibenzodioxins cytochrome P450 family 1 subfamily B member 1 Homo sapiens