Title : Oogenesis in Fundulus heteroclitus. VI. Establishment and verification of conditions for vitellogenin incorporation by oocytes in vitro.

Pub. Date : 1990 Jun

PMID : 2345348






5 Functional Relationships(s)
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1 Vitellogenic as well as maturational-stage oocytes were found to sequester X. laevis [32P]VTG from the medium, and incorporation was found to be linear with time for at least up to 12 hr. Phosphorus-32 a1-a Xenopus laevis
2 Once incorporated into the oocyte, [32P]VTG did not appear to undergo turnover. Phosphorus-32 a1-a Xenopus laevis
3 The effect of different [32P]VTG concentrations on incorporation indicated that the uptake mechanism was saturable. Phosphorus-32 a1-a Xenopus laevis
4 Unlabeled F. heteroclitus VTG and X. laevis VTG were also found to compete effectively with X. laevis [32P]VTG, whereas bovine serum albumin did not. Phosphorus-32 a1-a Xenopus laevis
5 Unlabeled F. heteroclitus VTG and X. laevis VTG were also found to compete effectively with X. laevis [32P]VTG, whereas bovine serum albumin did not. Phosphorus-32 a1-a Xenopus laevis