Title : Development of a heme sensor using fluorescently labeled heme oxygenase-1.

Pub. Date : 2013 Feb 1

PMID : 23068042






11 Functional Relationships(s)
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1 Development of a heme sensor using fluorescently labeled heme oxygenase-1. Heme heme oxygenase 1 Homo sapiens
2 To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. Heme heme oxygenase 1 Homo sapiens
3 To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. Heme heme oxygenase 1 Homo sapiens
4 To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. Heme heme oxygenase 1 Homo sapiens
5 To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. Heme heme oxygenase 1 Homo sapiens
6 To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. Heme heme oxygenase 1 Homo sapiens
7 To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. Heme heme oxygenase 1 Homo sapiens
8 To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. Heme heme oxygenase 1 Homo sapiens
9 To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. Heme heme oxygenase 1 Homo sapiens
10 Each of the three fluorescently labeled HO-1s exhibits a 1:1 binding stoichiometry and an absorption spectrum similar to that of the heme complex of the wild-type HO-1. Heme heme oxygenase 1 Homo sapiens
11 Titration of the labeled proteins with hemin resulted in fluorescence quenching in a hemin concentration-dependent manner, presumably due to an energy transfer from the fluorophore to the heme bound to HO-1. Heme heme oxygenase 1 Homo sapiens