Title : A simple method to quantitate IP-10 in dried blood and plasma spots.

Pub. Date : 2012

PMID : 22761744






4 Functional Relationships(s)
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1 AIM: To develop a robust and sensitive ELISA-based assay for IP-10 detection in plasma, dried blood spots (DBS) and dried plasma spots (DPS); to validate the ELISA in clinically relevant samples; and to assess the performance of the assay for detection of Cytomegalovirus (CMV) and M.tuberculosis specific immune responses. dbs C-X-C motif chemokine ligand 10 Homo sapiens
2 IP-10 was stabile in filter paper samples for at least 4 weeks at 37 C. The correlation between IP-10 detected in plasma, DPS and DBS samples was excellent (r(2)>0.97). dbs C-X-C motif chemokine ligand 10 Homo sapiens
3 IP-10 was stabile in filter paper samples for at least 4 weeks at 37 C. The correlation between IP-10 detected in plasma, DPS and DBS samples was excellent (r(2)>0.97). dbs C-X-C motif chemokine ligand 10 Homo sapiens
4 CONCLUSIONS: This newly developed assay is reliable for IP-10 quantification in plasma, DBS and DPS samples from antigen stimulated and non-stimulated whole blood. dbs C-X-C motif chemokine ligand 10 Homo sapiens