Pub. Date : 2012 May 15
PMID : 22542797
10 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Ascorbate stimulates endothelial nitric oxide synthase enzyme activity by rapid modulation of its phosphorylation status. | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 2 | Long-term exposure to ascorbate is known to enhance endothelial nitric oxide synthase (eNOS) activity by stabilizing the eNOS cofactor tetrahydrobiopterin (BH4). | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 3 | Long-term exposure to ascorbate is known to enhance endothelial nitric oxide synthase (eNOS) activity by stabilizing the eNOS cofactor tetrahydrobiopterin (BH4). | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 4 | Long-term exposure to ascorbate is known to enhance endothelial nitric oxide synthase (eNOS) activity by stabilizing the eNOS cofactor tetrahydrobiopterin (BH4). | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 5 | We investigated acute effects of ascorbate on eNOS function in primary (HUVEC) and immortalized human endothelial cells (EA.hy926), aiming to provide a molecular explanation for the rapid vasodilatation seen in vivo upon administration of ascorbate. | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 6 | Over a period of 4h, ascorbate steadily increased eNOS activity, although endothelial BH4 levels remained unchanged compared to untreated control cells. | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 7 | Immunoblot analyses revealed that as early as 5 min after treatment ascorbate dose-dependently increased phosphorylation at eNOS-Ser1177 and concomitantly decreased phosphorylation at eNOS-Thr495, a phosphorylation pattern indicative of increased eNOS activity. | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 8 | Immunoblot analyses revealed that as early as 5 min after treatment ascorbate dose-dependently increased phosphorylation at eNOS-Ser1177 and concomitantly decreased phosphorylation at eNOS-Thr495, a phosphorylation pattern indicative of increased eNOS activity. | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 9 | Immunoblot analyses revealed that as early as 5 min after treatment ascorbate dose-dependently increased phosphorylation at eNOS-Ser1177 and concomitantly decreased phosphorylation at eNOS-Thr495, a phosphorylation pattern indicative of increased eNOS activity. | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |
| 10 | In this report, we unravel a novel mechanism for how ascorbate rapidly activates eNOS independent of its effects on BH4 stabilization. | Ascorbic Acid | nitric oxide synthase 3 | Homo sapiens |