Title : Evolved stereoselective hydrolases for broad-spectrum G-type nerve agent detoxification.

Pub. Date : 2012 Apr 20

PMID : 22520752






1 Functional Relationships(s)
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1 Our selection for prevention of acetylcholinesterase inhibition also resulted in the complete reversion of PON1"s stereospecificity, from an enantiomeric ratio (E) < 6.3 x 10(-4) in favor of the R(P) isomer of a cyclosarin analog in wild-type PON1, to E > 2,500 for the S(P) isomer in an evolved variant. cyclohexyl methylphosphonofluoridate acetylcholinesterase (Cartwright blood group) Homo sapiens