Title : Enhancement of the proline and nitric oxide synthetic pathway improves fermentation ability under multiple baking-associated stress conditions in industrial baker's yeast.

Pub. Date : 2012 Apr 1

PMID : 22462683






3 Functional Relationships(s)
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1 We also found that expression of the proline-feedback inhibition-less sensitive mutant gamma-glutamyl kinase (Pro1-I150T) and the thermostable mutant Mpr1-F65L resulted in an enhanced fermentation ability of baker"s yeast in bread dough after freeze-thaw stress and air-drying stress, respectively. Proline glutamate 5-kinase Saccharomyces cerevisiae S288C
2 RESULTS: We constructed a self-cloned diploid baker"s yeast strain with enhanced proline and NO synthesis by expressing Pro1-I150T and Mpr1-F65L in the presence of functional Put1. Proline glutamate 5-kinase Saccharomyces cerevisiae S288C
3 We also demonstrated that baker"s yeast that enhances the proline and NO synthetic pathway by expressing the Pro1-I150T and Mpr1-F65L variants showed improved fermentation ability under multiple baking-associated stress conditions. Proline glutamate 5-kinase Saccharomyces cerevisiae S288C