Pub. Date : 1990 Nov 15
PMID : 2244925
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We show that 7-hydroxymethotrexate and dideazafolates require the prior binding of dUMP or its fluorinated derivative FdUMP to bind to thymidylate synthase, as does methotrexate. | 2'-deoxyuridylic acid | thymidylate synthetase | Homo sapiens |
| 2 | On the other hand, both dUMP and FdUMP exhibited a large cooperative effect on the affinity for thymidylate synthase of the inhibitors, and surprisingly, no significant difference was shown at this level between the natural substrate dUMP and its fluorinated derivative. | 2'-deoxyuridylic acid | thymidylate synthetase | Homo sapiens |
| 3 | On the other hand, both dUMP and FdUMP exhibited a large cooperative effect on the affinity for thymidylate synthase of the inhibitors, and surprisingly, no significant difference was shown at this level between the natural substrate dUMP and its fluorinated derivative. | 2'-deoxyuridylic acid | thymidylate synthetase | Homo sapiens |
| 4 | In the presence of FdUMP or dUMP, all the studied compounds except 7-hydroxymethotrexate exhibited a large negative enthalpy variation when binding to thymidylate synthase (from -44 to -91 kJ/mol). | 2'-deoxyuridylic acid | thymidylate synthetase | Homo sapiens |