Title : Simultaneous accumulation of proline and trehalose in industrial baker's yeast enhances fermentation ability in frozen dough.

Pub. Date : 2012 May

PMID : 22280966






1 Functional Relationships(s)
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1 In order to enhance the freeze tolerance of yeast cells, we constructed a self-cloning diploid baker"s yeast strain with simultaneous accumulation of proline, by expressing the PRO1-I150T allele, encoding the proline-feedback inhibition-less sensitive gamma-glutamyl kinase, and trehalose, by disrupting the NTH1 gene, encoding neutral trehalase. Proline glutamate 5-kinase Saccharomyces cerevisiae S288C