Title : Approaches to detect PARP-1 activation in vivo, in situ, and in vitro.

Pub. Date : 2011

PMID : 21870251






1 Functional Relationships(s)
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1 Since PARP-1 gene is expressed constitutively, its activation cannot be surmised from increased expression of its mRNA or protein, but by demonstrating the consequences of its catalytic -reaction which results in consumption of the substrate nicotinamide adenine dinucleotide (NAD(+)) and formation of three products, namely, polymer of ADP-ribose (pADPr or PAR), nicotinamide, and protons. Niacinamide poly(ADP-ribose) polymerase 1 Homo sapiens