Pub. Date : 2011 Aug
PMID : 21543742
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | An intramolecular transport metabolon: fusion of carbonic anhydrase II to the COOH terminus of the Cl(-)/HCO(3)(-)exchanger, AE1. | Bicarbonates | solute carrier family 4 member 1 (Diego blood group) | Homo sapiens |
| 2 | Anion exchanger 1 (AE1) is the plasma membrane Cl(-)/HCO(3)(-) exchanger of erythrocytes. | Bicarbonates | solute carrier family 4 member 1 (Diego blood group) | Homo sapiens |
| 3 | Anion exchanger 1 (AE1) is the plasma membrane Cl(-)/HCO(3)(-) exchanger of erythrocytes. | Bicarbonates | solute carrier family 4 member 1 (Diego blood group) | Homo sapiens |
| 4 | When expressed in transfected human embryonic kidney 293 cells, AE1.CAII had a similar Cl(-)/HCO(3)(-) exchange activity to AE1 alone, as assessed by the flux of H(+) equivalents (87 +- 4% vs. AE1) or rate of change of intracellular Cl(-) concentration (93 +- 4% vs. AE1), suggesting that CAII does not activate AE1. | Bicarbonates | solute carrier family 4 member 1 (Diego blood group) | Homo sapiens |
| 5 | Fusion of CAII to AE1 therefore reduces anion transport activity, but this reduction is compensated for during Cl(-)/HCO(3)(-) exchange by the presence of catalytically active CAII. | Bicarbonates | solute carrier family 4 member 1 (Diego blood group) | Homo sapiens |
| 6 | Overexpression of free CAII-V143Y acts in a dominant negative manner to reduce AE1-mediated HCO(3)(-) transport by displacement of endogenous CAII-wild type from its binding site on AE1. | Bicarbonates | solute carrier family 4 member 1 (Diego blood group) | Homo sapiens |
| 7 | Overexpression of free CAII-V143Y acts in a dominant negative manner to reduce AE1-mediated HCO(3)(-) transport by displacement of endogenous CAII-wild type from its binding site on AE1. | Bicarbonates | solute carrier family 4 member 1 (Diego blood group) | Homo sapiens |
| 8 | The bicarbonate transport activity of AE1 was inhibited by CAII-V143Y, whereas the activity of AE1.CAII was unaffected by CAII-V143Y, suggesting impaired transport activity upon displacement of functional CAII from AE1 but not AE1.CAII. | Bicarbonates | solute carrier family 4 member 1 (Diego blood group) | Homo sapiens |