Pub. Date : 2011 May
PMID : 21531298
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Feasibility of a novel positive feedback effect of 131I-promoted Bac-Egr1-hNIS expression in malignant glioma via baculovirus. | Iodine-131 | early growth response 1 | Homo sapiens |
| 2 | As Egr1 promoter is activated by 131I and may promote human NIS (hNIS) expression, hNIS also induces 131I uptake and activates Egr1, so the existence of a positive feedback effect of 131I-promoted Egr1-hNIS expression is possible. | Iodine-131 | early growth response 1 | Homo sapiens |
| 3 | To test 131I-promoted hNIS expression, human malignant glioma U87 cells were transfected with Bac-Egr1-hNIS, stimulated with or without 131I; the expression of hNIS protein was detected by immunofluorescence and flow cytometry test. | Iodine-131 | early growth response 1 | Homo sapiens |
| 4 | In addition, the uptake and efflux of 131I were determined after the incubation of Bac-Egr1-hNIS-transfected U87 cells with or without 131I. | Iodine-131 | early growth response 1 | Homo sapiens |
| 5 | CONCLUSION: Our results indicated that an improved transgene expression of 131I-stimulated hNIS in U87 cells using a baculovirus vector containing the Egr1 promoter is possible, and the increased expression of hNIS is responsible for a higher 131I uptake. | Iodine-131 | early growth response 1 | Homo sapiens |