Title : A screen for novel phosphoinositide 3-kinase effector proteins.

Pub. Date : 2011 Apr

PMID : 21263009






1 Functional Relationships(s)
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1 A secondary purification of these proteins, optimized using tandem pleckstrin homology domain containing protein-1 (TAPP-1), an established PtdIns(3,4)P(2) selective ligand, yields a fraction enriched in proteins of potentially similar lipid binding character that are identified by liquid chromatography-tandem MS. Thirdly, this approach is coupled to stable isotope labeling with amino acids in cell culture using differential isotope labeling of cells stimulated in the absence and presence of the PI 3-kinase inhibitor wortmannin. phosphoinositide-3,4-bisphosphate pleckstrin homology domain containing A1 Homo sapiens