Pub. Date : 1990 Aug
PMID : 2115115
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | To determine the structure of the CAR1 URS element, we performed a saturation mutagenesis. | Peptichemio | arginase | Saccharomyces cerevisiae S288C |
| 2 | Results of the mutagenic analysis indicated that the CAR1 URS was a 9-base-pair palindromic sequence, 5"-AGCCGCCGA-3". | Peptichemio | arginase | Saccharomyces cerevisiae S288C |
| 3 | These sequences were shown to support varying degrees of URS function in the expression vector assay, to bind protein as demonstrated by the gel shift assay, and to compete with a DNA fragment containing the CAR1 URS for protein binding. | Peptichemio | arginase | Saccharomyces cerevisiae S288C |
| 4 | These sequences were shown to support varying degrees of URS function in the expression vector assay, to bind protein as demonstrated by the gel shift assay, and to compete with a DNA fragment containing the CAR1 URS for protein binding. | Peptichemio | arginase | Saccharomyces cerevisiae S288C |
| 5 | These results indicate that the CAR1 URS element possesses the characteristics of a repressor binding site. | Peptichemio | arginase | Saccharomyces cerevisiae S288C |