Title : Vasoconstriction resulting from dynamic membrane trafficking of TRPM4 in vascular smooth muscle cells.

Pub. Date : 2010 Sep

PMID : 20610768






3 Functional Relationships(s)
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1 Using a cell surface biotinylation assay, we showed that PKC activation with phorbol 12-myristate 13-acetate (PMA) increased (approximately 3-fold) cell surface levels of TRPM4-GFP protein in <10 min. Tetradecanoylphorbol Acetate protein kinase C, alpha Rattus norvegicus
2 Using a cell surface biotinylation assay, we showed that PKC activation with phorbol 12-myristate 13-acetate (PMA) increased (approximately 3-fold) cell surface levels of TRPM4-GFP protein in <10 min. Tetradecanoylphorbol Acetate protein kinase C, alpha Rattus norvegicus
3 PMA-induced translocation of TRPM4 to the plasma membrane was independent of PKCalpha and PKCbeta activity but was inhibited by blockade of PKCdelta with rottlerin. Tetradecanoylphorbol Acetate protein kinase C, alpha Rattus norvegicus