Title : Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins.

Pub. Date : 2010 Jun

PMID : 20471598






1 Functional Relationships(s)
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1 Based on adsorption yield and residual activity of glutathione S-transferase (GST) after fusion with the PS19-6 peptide or its variants, it was found that the basic amino acid in the PS-tags, i.e., Arg was essential for the strong binding affinity of PS-tags in both the peptide and peptide-fused protein forms The aliphatic amino acids in PS19-6 and PS19-6L, such as Ile or Leu, were also effective. Leucine glutathione S-transferase kappa 1 Homo sapiens