Title : The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A.

Pub. Date : 2010 May

PMID : 20132841






6 Functional Relationships(s)
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Sentence
Compound Name
Protein Name
Organism
1 CsA inhibits isomerase activity of cellular-encoded cyclophilin proteins, of which cyclophilin A (CypA) in particular is required for HCV replication. Cyclosporine peptidylprolyl isomerase A Homo sapiens
2 Importantly, the NS5A-CypA interactions were sensitive to CsA in a dose-responsive manner and an isomerase mutant of CypA interacted with NS5A less efficiently than wild-type CypA. Cyclosporine peptidylprolyl isomerase A Homo sapiens
3 Importantly, the NS5A-CypA interactions were sensitive to CsA in a dose-responsive manner and an isomerase mutant of CypA interacted with NS5A less efficiently than wild-type CypA. Cyclosporine peptidylprolyl isomerase A Homo sapiens
4 Importantly, the NS5A-CypA interactions were sensitive to CsA in a dose-responsive manner and an isomerase mutant of CypA interacted with NS5A less efficiently than wild-type CypA. Cyclosporine peptidylprolyl isomerase A Homo sapiens
5 These findings correlate the anti-HCV properties of CsA with an ability of the compound to disrupt NS5A-CypA interactions in vitro and in vivo, whilst providing the basis for development of assay platforms suitable to screen compound libraries for novel inhibitors of the NS5A-CypA interaction. Cyclosporine peptidylprolyl isomerase A Homo sapiens
6 These findings correlate the anti-HCV properties of CsA with an ability of the compound to disrupt NS5A-CypA interactions in vitro and in vivo, whilst providing the basis for development of assay platforms suitable to screen compound libraries for novel inhibitors of the NS5A-CypA interaction. Cyclosporine peptidylprolyl isomerase A Homo sapiens