Pub. Date : 2009 Dec
PMID : 20044597
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 2 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 3 | METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 4 | METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 5 | METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 6 | METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 7 | Furthermore, in vitro enzyme kinetic studies established that 3BrPA has marked affinity to GAPDH. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 8 | CONCLUSION: GAPDH pyruvylation by 3BrPA affects its enzymatic function and is the primary intracellular target in 3BrPA mediated cancer cell death. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 9 | CONCLUSION: GAPDH pyruvylation by 3BrPA affects its enzymatic function and is the primary intracellular target in 3BrPA mediated cancer cell death. | bromopyruvate | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |