Title : Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death.

Pub. Date : 2009 Dec

PMID : 20044597






9 Functional Relationships(s)
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Protein Name
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1 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
2 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
3 METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
4 METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
5 METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
6 METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
7 Furthermore, in vitro enzyme kinetic studies established that 3BrPA has marked affinity to GAPDH. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
8 CONCLUSION: GAPDH pyruvylation by 3BrPA affects its enzymatic function and is the primary intracellular target in 3BrPA mediated cancer cell death. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
9 CONCLUSION: GAPDH pyruvylation by 3BrPA affects its enzymatic function and is the primary intracellular target in 3BrPA mediated cancer cell death. bromopyruvate glyceraldehyde-3-phosphate dehydrogenase Homo sapiens