Title : Cell-free synthesis of rat glucocorticoid receptor in rabbit reticulocyte lysate. In vitro synthesis of receptor in Mr 90,000 heat shock protein-depleted lysate.

Pub. Date : 1991 Jan 15

PMID : 1985950






3 Functional Relationships(s)
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1 Experimental evidence indicates that the purified nonactivated glucocorticoid receptor contains a single steroid-binding protein and two approximately 90-kDa nonsteroid-binding subunits identified as heat shock protein (hsp) 90. Steroids nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus
2 The cell-free synthesized glucocorticoid receptor is able to bind steroid and can be activated further to the DNA-binding form. Steroids nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus
3 To test the hypothesis of an active role played by hsp90 in the stabilization of a competent steroid-binding conformation of the glucocorticoid receptor, we have synthesized the receptor in a reticulocyte lysate that has been depleted of hsp90 by immunoadsorption with AC88 anti-hsp90. Steroids nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus