Title : Effects of citraconylation on enzymatic modification of human proinsulin using trypsin and carboxypeptidase B.

Pub. Date : 2009 Jul-Aug

PMID : 19572292






2 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 After the enzymatic hydrolysis of the citraconylated proinsulin, 100% of lysine residues can be decitraconylated and restored by adjusting pH to 2-3 at 25 degrees C. Combination of hydrogen peroxide addition and citraconylation of proinsulin expressed in recombinant Escherichia coli remarkably improved the conversion yield of insulin from 52.7 to 77.7%. Hydrogen Peroxide insulin Homo sapiens
2 After the enzymatic hydrolysis of the citraconylated proinsulin, 100% of lysine residues can be decitraconylated and restored by adjusting pH to 2-3 at 25 degrees C. Combination of hydrogen peroxide addition and citraconylation of proinsulin expressed in recombinant Escherichia coli remarkably improved the conversion yield of insulin from 52.7 to 77.7%. Hydrogen Peroxide insulin Homo sapiens