Pub. Date : 2009 Oct
PMID : 19286649
2 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | ATP citrate lyase (ACL) is a cytosolic enzyme that catalyzes the synthesis of acetyl-CoA and oxaloacetate using citrate, CoA, and ATP as substrates and Mg(2+) as a necessary cofactor. | Citric Acid | ATP citrate lyase | Homo sapiens |
| 2 | The current assay consists of 1) incubation of ACL enzyme with [(14)C]citrate and other substrates/cofactors CoA, ATP, and Mg(2+), 2) EDTA quench, 3) addition of MicroScint-O, the agent that specifically detects product [(14)C]acetyl-CoA, and 4) detection of signal by TopCount. | Citric Acid | ATP citrate lyase | Homo sapiens |