Title : Assessment of protein phosphatase in a re-usable rapid assay format in detecting microcystins and okadaic acid as a precursor to biosensor development.

Pub. Date : 2008 Dec 1

PMID : 18812183






5 Functional Relationships(s)
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1 Under optimised immobilised enzyme assay conditions, okadaic acid (OA) and microcystin LR (MC-LR) inhibited Upstate Biotechnology PP (PP-2A), with IC50 values of 12.5 and 11nM respectively. cyanoginosin LR protein phosphatase 2 phosphatase activator Homo sapiens
2 Under optimised immobilised enzyme assay conditions, okadaic acid (OA) and microcystin LR (MC-LR) inhibited Upstate Biotechnology PP (PP-2A), with IC50 values of 12.5 and 11nM respectively. cyanoginosin LR protein phosphatase 2 phosphatase activator Homo sapiens
3 Of the three immobilised enzyme systems, PP-2A showed greatest sensitivity to OA and MC-LR followed by rec PP-1 and PP-2Ana. cyanoginosin LR protein phosphatase 2 phosphatase activator Homo sapiens
4 In assessments for re-usability (determined by removal of > or =70% OA or MC-LR inhibition of PP-2A by washing), <50% of the original activity remained after 20 washings. cyanoginosin LR protein phosphatase 2 phosphatase activator Homo sapiens
5 The LoD of immobilised PP-2A to MC-LR meets the WHO guideline of 1microgl(-1) for drinking water, and the sensitivity to OA (3.5microgl(-1)) would allow detection of DSP during the peak of some phytoplankton blooms. cyanoginosin LR protein phosphatase 2 phosphatase activator Homo sapiens