Title : Subtle differences in active site structure between bovine and human thrombins: ESR and fluorescence studies.

Pub. Date : 1991 Jan 23

PMID : 1850875






1 Functional Relationships(s)
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1 The two active site directed fluorophores, dansyl fluoride and p-nitrophenyl anthranilate showed differences in both lambda emmax and lambda exmax of the complexes with bovine and human-alpha-thrombin, respectively, Several of the effects observed i.e., ligand binding (indole or benzamidine) and the subtle hydrophobic interactions between the nitroxide moiety and the protein active site would be difficult to assess from an x-ray structure determination alone. Hydroxylamine coagulation factor II, thrombin Homo sapiens