Title : A short human and mouse MLR assay utilizing lymphokine (IL-2, IL-3) secretion as an early activation event.

Pub. Date : 1991 May

PMID : 1827689






1 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 We here describe a shorter assay in which IL-2 and IL-3 secretion during MLR was assessed by adding the respective lymphokine-dependent cell lines either to the MLR supernatants or directly to the original MLR cultures, using the colorimetric (3-[4,5 Dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide) (MTT) technique or the 3H-thymidine incorporation assay. 3h-thymidine interleukin 3 Homo sapiens