Pub. Date : 2008 Apr
PMID : 18022818
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Molecular mechanisms of p21 and p27 induction by 3-methylcholanthrene, an aryl-hydrocarbon receptor agonist, involved in antiproliferation of human umbilical vascular endothelial cells. | Methylcholanthrene | H3 histone pseudogene 16 | Homo sapiens |
| 2 | Herein, pretreatment of HUVECs with p21 or p27 small interfering (si)RNA reduced 3MC-induced elimination of [(3)H]thymidine incorporation, demonstrating their essential roles in the antiproliferation of HUVECs. | Methylcholanthrene | H3 histone pseudogene 16 | Homo sapiens |
| 3 | The molecular mechanisms of p21 and p27 involved in the antiproliferative effects of 3MC were elucidated in this study. | Methylcholanthrene | H3 histone pseudogene 16 | Homo sapiens |
| 4 | Interestingly, 3MC-mediated p21 and p27 inductions were eliminated by resveratrol, an AhR antagonist, suggesting their AhR dependency, further confirmed by AhR siRNA. | Methylcholanthrene | H3 histone pseudogene 16 | Homo sapiens |
| 5 | Among the relevant pathways, p38MAPK activation sustained the levels of p21 and p27 induced by 3MC, which was eliminated by AhR antagonists and N-acetylcysteine (NAC), an antioxidant. | Methylcholanthrene | H3 histone pseudogene 16 | Homo sapiens |
| 6 | 3MC concentration-dependently enhanced not only the consensus dioxin-responsive element (DRE)-driven luciferase activity, but also the binding activity of the AhR to the putative DRE derived from the p21 and p27 promoters. | Methylcholanthrene | H3 histone pseudogene 16 | Homo sapiens |
| 7 | A deletion of the DRE (-285/-270) in p21 (-2,300/+8) only partially alleviated the 3MC-induced luciferase activity unless NAC was added, suggesting that there may be a DRE-independent mechanism associated with oxidative stress. | Methylcholanthrene | H3 histone pseudogene 16 | Homo sapiens |
| 8 | Our study demonstrated that both the functional DRE and the phosphorylation of p38MAPK are essential for the induction of p21 and p27, resulting in the antiproliferative action of 3MC in HUVECs. | Methylcholanthrene | H3 histone pseudogene 16 | Homo sapiens |