Pub. Date : 2007 May 11
PMID : 17376479
11 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Unlike other LacI-GalR proteins, CcpA is activated to bind DNA by binding the phosphoproteins HPr-Ser46-P or Crh-Ser46-P. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 2 | However, fine regulation of CCR is accomplished by the small molecule effectors, glucose 6-phosphate (G6P) and fructose 1,6-bisphosphate (FBP), which somehow enhance CcpA-(HPr-Ser46-P) binding to DNA. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 3 | To understand the fine-tuning mechanism of these effectors, we solved the structures of the CcpA core, DeltaCcpA, which lacks the N-terminal DNA-binding domain, in complex with HPr-Ser46-P and G6P or FBP. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 4 | This suggests that one role of the adjunct corepressors is to buttress the DNA-binding conformation effected by the binding of HPr-Ser46-P to the CcpA dimer N subdomains. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 5 | However, the structures reveal that an unexpected function of adjunct corepressor binding is to bolster cross interactions between HPr-Ser46-P residue Arg17 and residues Asp69 and Asp99 of the other CcpA subunit. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 6 | These cross contacts, which are weak or not present in the CcpA-(Crh-Ser46-P) complex, stimulate the CcpA-(HPr-Ser46-P)-DNA interaction specifically. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 7 | These cross contacts, which are weak or not present in the CcpA-(Crh-Ser46-P) complex, stimulate the CcpA-(HPr-Ser46-P)-DNA interaction specifically. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 8 | Thus, stabilization of the closed conformation and bolstering of cross contacts between CcpA and its other corepressor, HPr-Ser46-P, provide a molecular explanation for how adjunct corepressors G6P and FBP enhance the interaction between CcpA-(HPr-Ser46-P) and cognate DNA. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 9 | Thus, stabilization of the closed conformation and bolstering of cross contacts between CcpA and its other corepressor, HPr-Ser46-P, provide a molecular explanation for how adjunct corepressors G6P and FBP enhance the interaction between CcpA-(HPr-Ser46-P) and cognate DNA. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 10 | Thus, stabilization of the closed conformation and bolstering of cross contacts between CcpA and its other corepressor, HPr-Ser46-P, provide a molecular explanation for how adjunct corepressors G6P and FBP enhance the interaction between CcpA-(HPr-Ser46-P) and cognate DNA. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |
| 11 | Thus, stabilization of the closed conformation and bolstering of cross contacts between CcpA and its other corepressor, HPr-Ser46-P, provide a molecular explanation for how adjunct corepressors G6P and FBP enhance the interaction between CcpA-(HPr-Ser46-P) and cognate DNA. | 2-chloro-N(6)cyclopentyladenosine | haptoglobin-related protein | Homo sapiens |