Title : [The chemiluminescent determination of cholesterol].

Pub. Date : 1991

PMID : 1721947






1 Functional Relationships(s)
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1 The method is based on coupled enzymatic reactions in two steps: (1) esterified cholesterol hydrolysis by cholesterol esterase in the presence of sodium cholate and (2) cholesterol oxidation by cholesterol oxidase and chemiluminescent assay of the released hydrogen peroxide in peroxidase reaction with luminol and p-iodophenol. Sodium Cholate carboxyl ester lipase Homo sapiens