Title : Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography.

Pub. Date : 2007 Apr 10

PMID : 17210183






1 Functional Relationships(s)
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1 Blue-Sepharose is often used to remove bovine serum albumin (BSA) from samples, but when we applied BSA to Blue-Sepharose in 20 mM phosphate, pH 7.0, 50%-60% of the protein flowed through the column; however, complete binding of BSA was achieved by the addition of 2 M ammonium sulfate (AS) to the column equilibration buffer and the sample. Sepharose albumin Homo sapiens