Pub. Date : 2007 Jan
PMID : 17166886
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | GADD153 mediates celecoxib-induced apoptosis in cervical cancer cells. | Celecoxib | DNA damage inducible transcript 3 | Homo sapiens |
| 2 | Screening of a microarray cDNA-chip containing 225 different genes revealed that growth arrest and DNA damage inducible gene (GADD153), a transcription factor involved in apoptosis, showed the strongest differential expression following celecoxib treatment in all three cervical cancer cell lines. | Celecoxib | DNA damage inducible transcript 3 | Homo sapiens |
| 3 | Notably, siRNA-induced silencing of GADD153 suppressed celecoxib-induced apoptosis in all the three cell lines, and exogenous expression of GADD153 triggered apoptosis in cervical cancer cells in the absence of other apoptotic stimuli. | Celecoxib | DNA damage inducible transcript 3 | Homo sapiens |
| 4 | A luciferase reporter gene assay and mRNA stability tests revealed that expression of GADD153 was regulated at both the transcriptional and post-transcriptional levels following celecoxib treatment. | Celecoxib | DNA damage inducible transcript 3 | Homo sapiens |
| 5 | The region between -649 and -249, containing an intact C/EBP-ATF binding site, was required for the basal activity and celecoxib-induced stimulation of GADD153 promoter activity. | Celecoxib | DNA damage inducible transcript 3 | Homo sapiens |
| 6 | Also, mRNA stability test showed that celecoxib prolonged the half-life of GADD153 mRNA. | Celecoxib | DNA damage inducible transcript 3 | Homo sapiens |
| 7 | These novel findings collectively suggest that GADD153 may play a key role in celecoxib-induced apoptosis in cervical cancer cells by regulating the expression of proapoptotic proteins such as Bak. | Celecoxib | DNA damage inducible transcript 3 | Homo sapiens |