Pub. Date : 2006 Nov 10
PMID : 16934821
2 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | Polymerase chain reaction (PCR), lambda exonuclease digestion combined with (AC)(5)-Sepharose DNA affinity chromatography were used to produce c-jun promoter with a (GT)(5) tail at each 3" end. | Sepharose | Jun proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |
2 | In solution, tailed c-jun promoter (60 nM) and competitor poly dI:dC (30 ng/microl) was incubated with crude HEK293 nuclear extract to form a large protein-promoter complex, and the complex was then trapped by (AC)(5)-Sepharose by centrifugation or on a column. | Sepharose | Jun proto-oncogene, AP-1 transcription factor subunit | Homo sapiens |