Title : Promoter trapping of c-jun promoter-binding transcription factors.

Pub. Date : 2006 Nov 10

PMID : 16934821






2 Functional Relationships(s)
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1 Polymerase chain reaction (PCR), lambda exonuclease digestion combined with (AC)(5)-Sepharose DNA affinity chromatography were used to produce c-jun promoter with a (GT)(5) tail at each 3" end. Sepharose Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens
2 In solution, tailed c-jun promoter (60 nM) and competitor poly dI:dC (30 ng/microl) was incubated with crude HEK293 nuclear extract to form a large protein-promoter complex, and the complex was then trapped by (AC)(5)-Sepharose by centrifugation or on a column. Sepharose Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens