Pub. Date : 1975 Aug
PMID : 169122
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In cultures treated with cycloheximide (10 mug/ml) or proline (6.3 mM) the initial binding of [2,3-3H-Pro]TRH to receptors, measured after 1 h, was 97% or 102% of control. | Proline | thyrotropin releasing hormone | Rattus norvegicus |
| 2 | However, the incorporation of label from [2,3-3H-Pro]TRH into an acid-precipitable product after 22 h was inhibited by 81 and 74% by cycloheximide (1 mug/ml) and proline (2.5 mM). | Proline | thyrotropin releasing hormone | Rattus norvegicus |
| 3 | Formation of [2,3-3H] proline from [2,3-?3H-Pro] TRH was demonstrated by thin layer chromatography; the percentage of non-protein radioactivity with an Rf of proline increased from 20 to 80% in GH3 cells incubated 1 or 24 h with [2,3-3H-Pro]TRH. | Proline | thyrotropin releasing hormone | Rattus norvegicus |
| 4 | Formation of [2,3-3H] proline from [2,3-?3H-Pro] TRH was demonstrated by thin layer chromatography; the percentage of non-protein radioactivity with an Rf of proline increased from 20 to 80% in GH3 cells incubated 1 or 24 h with [2,3-3H-Pro]TRH. | Proline | thyrotropin releasing hormone | Rattus norvegicus |
| 5 | We conclude that after binding to receptors on GH3 cells, TRH is slowly metabolized to its constituent amino acids, and the products [2,3-3H]proline or [14C]histidine are incorporated into newly synthesized proteins. | Proline | thyrotropin releasing hormone | Rattus norvegicus |