Pub. Date : 1968 Jun
PMID : 16656864
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | With respect to cofactor requirements, NADH, and FMNH(2) were equally effective as electron donors for nitrate reductase obtained from leaves of maize, marrow, and spinach, when the cofactors were supplied in optimal concentrations. | NAD | nitrate reductase [NADH] 1 | Zea mays |
| 2 | Unity of NADH to FMNH(2) activities were obtained during: A) purification procedures (4 step, 30-fold); B) induction of nitrate reductase in corn seedlings with nitrate; and C) inactivation of nitrate reductase in intact or excised corn seedlings. | NAD | nitrate reductase [NADH] 1 | Zea mays |
| 3 | Unity of NADH to FMNH(2) activities were obtained during: A) purification procedures (4 step, 30-fold); B) induction of nitrate reductase in corn seedlings with nitrate; and C) inactivation of nitrate reductase in intact or excised corn seedlings. | NAD | nitrate reductase [NADH] 1 | Zea mays |
| 4 | The NADH- and FMNH(2)-dependent activities were not additive.A half-life for nitrate reductase of approximately 4 hours was estimated from the inactivation studies with excised corn seedlings. | NAD | nitrate reductase [NADH] 1 | Zea mays |
| 5 | This was verified by reactivation with exogenous cysteine.Based on these current findings, and previous work, it is concluded that nitrate reductase is a single moiety with the ability to utilize either NADH or FMNH(2) as cofactor. | NAD | nitrate reductase [NADH] 1 | Zea mays |
| 6 | However the high concentration of FMNH(2) required for optimal activity suggests that in vivo NADH is the electron donor and that nitrate reductase in higher plants should be designated NADH:nitrate reductase (E.C. | NAD | nitrate reductase [NADH] 1 | Zea mays |
| 7 | However the high concentration of FMNH(2) required for optimal activity suggests that in vivo NADH is the electron donor and that nitrate reductase in higher plants should be designated NADH:nitrate reductase (E.C. | NAD | nitrate reductase [NADH] 1 | Zea mays |
| 8 | However the high concentration of FMNH(2) required for optimal activity suggests that in vivo NADH is the electron donor and that nitrate reductase in higher plants should be designated NADH:nitrate reductase (E.C. | NAD | nitrate reductase [NADH] 1 | Zea mays |