Title : An enzyme-coupled continuous spectrophotometric assay for S-adenosylmethionine-dependent methyltransferases.

Pub. Date : 2006 Mar 15

PMID : 16460659






2 Functional Relationships(s)
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1 In this assay, S-adenosyl-L-homocysteine (AdoHcy/SAH), the transmethylation product of AdoMet-dependent methyltransferases, is hydrolyzed to S-ribosylhomocysteine and adenine by recombinant S-adenosylhomocysteine/5"-methylthioadenosine nucleosidase (SAHN/MTAN, EC 3.2.2.9). S-Adenosylhomocysteine acyl-CoA synthetase medium-chain family member 3 Rattus norvegicus
2 In this assay, S-adenosyl-L-homocysteine (AdoHcy/SAH), the transmethylation product of AdoMet-dependent methyltransferases, is hydrolyzed to S-ribosylhomocysteine and adenine by recombinant S-adenosylhomocysteine/5"-methylthioadenosine nucleosidase (SAHN/MTAN, EC 3.2.2.9). S-Adenosylhomocysteine acyl-CoA synthetase medium-chain family member 3 Rattus norvegicus