Title : Specific translocation of protein kinase Calpha to the plasma membrane requires both Ca2+ and PIP2 recognition by its C2 domain.

Pub. Date : 2006 Jan

PMID : 16236797






4 Functional Relationships(s)
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1 Specific translocation of protein kinase Calpha to the plasma membrane requires both Ca2+ and PIP2 recognition by its C2 domain. Phosphatidylinositol 4,5-Diphosphate protein kinase C alpha Homo sapiens
2 We find that Ca2+-activated PKCalpha and its isolated C2 domain localize exclusively to the plasma membrane in vivo and that a plasma membrane lipid, phosphatidylinositol-4,5-bisphosphate (PIP2), dramatically enhances the Ca2+-triggered binding of the C2 domain to membranes in vitro. Phosphatidylinositol 4,5-Diphosphate protein kinase C alpha Homo sapiens
3 Similarly, a hybrid construct substituting the PKCalpha Ca2+-binding loops (CBLs) and PIP2 binding site (beta-strands 3-4) into a different C2 domain exhibits native Ca2+-triggered targeting to plasma membrane and recognizes PIP2. Phosphatidylinositol 4,5-Diphosphate protein kinase C alpha Homo sapiens
4 Similarly, a hybrid construct substituting the PKCalpha Ca2+-binding loops (CBLs) and PIP2 binding site (beta-strands 3-4) into a different C2 domain exhibits native Ca2+-triggered targeting to plasma membrane and recognizes PIP2. Phosphatidylinositol 4,5-Diphosphate protein kinase C alpha Homo sapiens