Pub. Date : 2005 Oct
PMID : 16215634
2 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In general, replacement of B8Gly by other amino acids causes significant detriment to the foldability of single-chain insulin: the conformations of the three B8 mutants are essentially different from that of wild-type molecules as revealed by circular dichroism; their disulfide stabilities in redox buffer are significantly decreased; their in vitro refolding efficiencies are decreased approximately two folds; the structural stabilities of the mutants with Ser or Thr substitution are decreased significantly, while Leu substitution has little effect as measured by equilibrium guanidine denaturation. | Threonine | insulin | Homo sapiens |
| 2 | But Thr or Leu replacement produces significant detriment: the receptor-binding potencies of the two mutants are less than 0.2% of native insulin. | Threonine | insulin | Homo sapiens |