Title : Expression of lipase-solubilized bovine liver microsomal cytochrome b5 in Escherichia coli as a glutathione S-transferase fusion protein (GST-cyt b5).

Pub. Date : 2006 Feb

PMID : 16146697






1 Functional Relationships(s)
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1 The GST-cyt b5 fusion protein can be matured in vivo as a holoprotein with heme incorporated into cyt b5 during the fermentation, and the purification procedures were simplified by using a one-step affinity column chromatography with glutathione-agarose gel. Sepharose cytochrome b5 type A Bos taurus