Pub. Date : 2005 Oct 21
PMID : 16109712
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | To gain insight into these mechanisms, human DAT was purified in large amounts using a two-step affinity chromatography procedure from untreated HeLa cells or cells treated with phorbol 12-myristate 13-acetate (PMA). | Tetradecanoylphorbol Acetate | solute carrier family 6 member 3 | Homo sapiens |
| 2 | To gain insight into these mechanisms, human DAT was purified in large amounts using a two-step affinity chromatography procedure from untreated HeLa cells or cells treated with phorbol 12-myristate 13-acetate (PMA). | Tetradecanoylphorbol Acetate | solute carrier family 6 member 3 | Homo sapiens |
| 3 | Mass spectrometric analysis of purified DAT complexes revealed the presence of several proteins, among which ubiquitin was particularly abundant in the PMA-treated sample. | Tetradecanoylphorbol Acetate | solute carrier family 6 member 3 | Homo sapiens |
| 4 | Western blotting of highly purified DAT protein confirmed constitutive ubiquitylation of DAT and a dramatic increase in DAT ubiquitylation in cells treated with PMA. | Tetradecanoylphorbol Acetate | solute carrier family 6 member 3 | Homo sapiens |